| Enzyme: | TRD1 (m4C) | TRD2 (m6A) |
| S.PacII | 1–145 | 193–342 |
| S.Mma5219I | 1–150 | (not swapped) |
| S.Asu14238II | 1–148 | (not swapped) |
| S.DbaKI | 1–145 | 198–356 |
| S.Apa12260I | 1–150 | (not swapped) |
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