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. 2016 Jun 30;44(19):9131–9141. doi: 10.1093/nar/gkw586

Figure 2.

Figure 2.

H1 CTD FRET during salt-dependent folding of nucleosome arrays. (A) Total FRET increases upon salt-dependent folding of 12-mer nucleosome arrays containing Cy3/Cy5-labeled H1 G101C/K195C (squares). FRET response of the labeled H1 in the absence of arrays over the same range of salt concentrations is shown (circles). (B) Inter-molecular FRET between labeled H1s increases upon salt-dependent folding of nucleosome arrays. A 50/50 mixture of Cy3-labeled and Cy5 labeled H1 G101C/K195C was bound to 12-mer nucleosome arrays and FRET efficiency determined over the range of salt concentrations shown.