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. 2016 Nov 7;7:1611. doi: 10.3389/fpls.2016.01611

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Copyright © 2016 Dobrenel, Mancera-Martínez, Forzani, Azzopardi, Davanture, Moreau, Schepetilnikov, Chicher, Langella, Zivy, Robaglia, Ryabova, Hanson and Meyer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Kinetics of variations in RPS6 phosphorylation following AZD-8055 or sucrose addition. Total protein extracts obtained from seedlings were separated by SDS-PAGE and blotted onto a membrane. After incubation with the phospho-specific antibody against RPS6 Ser240 (P-RPS6) blots were revealed by a secondary antibody linked to HRP activity and imaged with a CCD camera. (A) Kinetics of the inhibition of RPS6 Ser240 phosphorylation by AZD 8055. Seven day-old seedlings were either mock (DMSO) or AZD-8055 (2 μM) treated and then harvested at the indicated time. (B) Kinetics of the induction of RPS6 Ser240 phosphorylation by sucrose. Seven day-old seedlings were transferred in sugar-free medium for 24 h and then either mock (0) or sucrose (0.5%) treated. Seedlings were harvested at the indicated time. Bottom panels show Ponceau Red staining of the membranes.