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. Author manuscript; available in PMC: 2016 Nov 8.
Published in final edited form as: J Stem Cell Regen Biol. 2016 May 27;2(2):10.15436/2471-0598.16.013. doi: 10.15436/2471-0598.16.013

Figure 1. Native salivary gland tissue exhibits neuronal organization around salivary epithelial structures.

Figure 1

Immunofluorescent images taken at 20× magnification reveal a distribution of neurons on the basolateral side of salivary epithelial structures, including acini (triangles), striated ducts (arrows), and excretory ducts (asterisks). Scale bars are 20 µm. (A) ZO-1 (green) is observed lining luminal spaces in acinar and ductal structures. (B) B-3-tubulin staining (red) indicative of neuronal cytoskeleton is distributed basolateral to epithelial structures. (C) TO-PRO (blue) staining indicates cell nuclei. (D) Overlay image.