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Composition of growth medium used for all culture conditions
| Material | Vendor |
|---|---|
| Ham’s F12/DMEM (1:1) | Thermo Fisher |
| FBS (2.5%) | Thermo Fisher |
| Insulin-Transferrin-Selenite (10 µg/mL) | Sigma |
| Retinoic Acid (0.1 µM) | Sigma |
| EGF (80 ng/mL) | Sigma |
| T3 (3,3′,5 Triiodothyronine) | Sigma |
| Hydrocortisone (1.1 µM) | Sigma |
| Glutamine (5 mM) | Thermo Fisher |
| Gentamicin (50 µg/ml) | Sigma |
