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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Biol Bull. 2016 Aug;231(1):5–13. doi: 10.1086/689587

Figure 3.

Figure 3

Comparison of the electron multiplying charge-coupled device (EMCCD) and scientific complementary metal-oxide-semiconductor (sCMOS) cameras for use with a spinning disk confocal microscope. Adjacent cells were imaged, making use of either an EMCCD or sCMOS camera, using different tube lenses such that the pixel size in the image plane was the same for both (16-μm pixels for the EMCCD, with a 125-mm tube lens; 6.5-μm pixels for the sCMOS, with a 50-mm tube lens). (Reprinted from fig. 9.8, panel b, of Oreopoulos, J., et al., 2013, Methods Cell Biol. 123: 153–175, with permission from Elsevier.) from Photometrics can execute a complicated noise reduction algorithm on the FPGA of the camera before the image reaches the computer.