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. Author manuscript; available in PMC: 2017 Oct 19.
Published in final edited form as: Bioconjug Chem. 2016 Sep 14;27(10):2407–2417. doi: 10.1021/acs.bioconjchem.6b00377

Figure 5.

Figure 5

Evaluation of probe 3 binding to hMMP-12-FITC anchored at the HeLa cells surface. (A) hMMP-12-FITC activity was assayed at 25°C in presence of a fluorogenic substrate (Mca-GluProGluLeuGluGluDpa) at 4μM. Probe 3 at three different concentrations (1nM, 10 nM and 100nM) was incubated at 4°C for 15 min before measuring the activity during 30 min at 25°C. (B) Confocal microscopy of HeLa cells incubated with hMMP-12-FITC (green colour) and probe 3 (red colour). Blue (491 nm) and red (642 nm) lasers were used for excitation of hMMP-12-FITC and probe 3 with a Cy5.5-6SIDCC dye respectively. Bandpass filters of 525/45 nm and 692/40 nm allowed the detection of FITC and Cy5.5-6SIDCC respectively.