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. Author manuscript; available in PMC: 2016 Nov 8.
Published in final edited form as: J Chromatogr Sep Tech. 2016 Sep 21;7(5):336. doi: 10.4172/2157-7064.1000336

Table 1.

Gradient used on the loading and eluting pumps for the TFLC-LS/MS analysis of MMA.

Step Time (s) Flow (mL/min) %A %B %C Flow (mL/Min) Grad. %A %B
1 30 3.00 100 - - 0.8 Step 92 8
21 30 0.90 100 - - 0.2 Step 92 8
32 90 2.00 - 50 50 0.8 Step 92 8
4 20 2.00 - 50 50 0.8 Step 50 50
5 40 3.50 - 100 - 0.8 Step 50 50
6 20 3.50 - 100 - 0.8 Step 92 8
7 120 2.00 100 - - 0.8 Ramp 92 8
8 10 3.00 100 - - 0.8 Ramp 92 8
1

During this step valve “A” and valve “B” are in line and the analyte is transferred from the turbulent flow column to the analytical column.

2

Data Acquisition window: 1.5–3.0 minutes.