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. 2016 Oct 4;172(3):1708–1719. doi: 10.1104/pp.16.01332

Figure 1.

Figure 1.

OsHAC1;1 and OsHAC1;2 encode arsenate reductases. A, Expression of OsHAC1;1 or OsHAC1;2 suppresses the As(V) sensitivity of the E. coli mutant lacking the arsC arsenate reductase. Strains were grown at 16°C and cell density measured at OD600 nm after exposure to 1 mm As(V) for 0 to 72 h. Wild type = E. coli wild type (W3110); ΔarsC = arsC mutant in WC3110; Vector = empty pET29a; arsC-HAC1;1 = pET-29a vector containing OsHAC1;1; ΔarsC-HAC1;2 = pET-29a vector containing OsHAC1;2. B, Production of As(III) in LB medium after E. coli expressing the empty vector (EV), OsHAC1;1 or OsHAC1;2 was exposed to 10 µM As(V). n.d., not detected.