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Fe accumulation and ROS generation in the roots of the transgenic apple plants and the wild-type control under iron-sufficient (+Fe) and -deficient (−Fe+Frz) conditions. A, Perls/DAB staining of Fe distribution in the roots. Scale bar, 200 μm. B, Fe staining (Perls only) in the roots. Scale bar, 200 μm. C, C-H2DCFDH staining of ROS in the roots. Scale bar, 200 μm. D, NBT staining of superoxide in the roots. Scale bar, 200 μm.
