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. 2016 Sep 22;172(3):1973–1988. doi: 10.1104/pp.16.01323

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Figure 7. — Rhizosphere acidification, Fe distribution, and ROS generation in the roots of the 35S::MdbHLH104-GFP transgenic apple plants and the wild-type control under iron-sufficient (+Fe) and -deficient (−Fe+Frz) conditions. A, Rhizosphere acidification of the wild-type control and transgenic apple lines. The yellow color around the roots stained with Bromocresol Purple indicates acidification. B and C, Phenotypic comparison of Fe (Perls/DAB) and ROS (C-H2DCFDH) staining in the roots of the transgenic apple plants as indicated. Scale bar, 200 μm.