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. 2016 Sep 22;172(3):1973–1988. doi: 10.1104/pp.16.01323

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Figure 8. — MdBT2 regulates H⁺-ATPase activity and iron uptake. A, Acidification of transgenic apple calli. After allowing the calli to grow in the iron-deficient media for 5 d, the calli were exposed to the pH indicator dye Bromocresol Purple for 24 h. Acidification is indicated as the yellow color around the apple calli. B, PM H⁺-ATPase activity in vesicles isolated from the wild-type control, 35S::MdBT2 and 35S::MdBT1/2-anti transgenic apple calli that had been treated with (+Fe) or without (−Fe+Frz) iron for 7 d. C, Visualization of ferrous iron in the wild-type control, 35S::MdBT2-GFP and 35S::MdBT1/2-anti transgenic apple calli with FerroZine under iron-sufficient (+Fe) or iron-deficient (−Fe+Frz) conditions. The resultant Fe(II) was trapped by FerroZine to produce a red product. D, Fe content of the wild-type control, 35S::MdBT2-GFP and 35S::MdBT1/2-anti transgenic apple calli treated with (+Fe) or without (−Fe+Frz) Fe-EDTA for 7 d. The data represent the means ± sd of three independent experiments. DW, Dry weight.