Figure 7.

Membrane release of GmNTLs. A, Transiently transformed Arabidopsis protoplasts assayed 5 h after exposure to low temperature or H₂O₂. The GFP (green) and mCherry (magenta) signals mark the nucleus/cytoplasm and the ER, respectively. Cold, Protoplasts exposed to 4°C for 15 min; H₂O₂, protoplasts exposed to 10 mm H₂O₂ for 15 min. Arrows indicate the nuclei. Bars = 20 μm. B, The inducibility of alternative GmNTL transcripts in the roots of plants exposed to either 200 mm NaCl or 100 µm ABA. The data shown are means ± se (n = 3). Statistically significant differences (*, P < 0.05; **, P < 0.01; ***, P < 0.001) in transcript abundance are shown between treated and mock-treated samples.