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. 2016 Sep 12;172(3):1899–1910. doi: 10.1104/pp.16.01189

Figure 2.

Figure 2.

Metal transport activity assay of HvNramp5 in yeast. A, Functional complementation for Mn uptake in the strain ∆smf1. B, Functional complementation for Fe uptake in the strain DDY4 (∆fet3fet4). C, Sensitivity test to Cd toxicity in the strain BY4741. Different yeast strains carrying HvNramp5, HvIRT1, or empty vector (pYES2) were cultured on the plate with or without Mn-chelator EGTA (A), Fe-chelator BPDS (B), or Cd (C). Eight microliters of cell suspension with an OD600nm of 0.2 and three serial 1:10 dilutions were spotted and incubated at 30°C for 3 to 5 d. D to E, Cd uptake in liquid medium. Yeast BY4741 carrying HvNramp5, HvIRT1, or empty vector (pYES2) was cultured in a liquid medium containing 2% Glc (D) or Gal (E) in the presence of 5 µm CdCl2 for 4 h. The Cd concentration in the yeast was determined with ICP-MS after digestion. Data are means ± sd of three biological replicates and a different small letter indicates significant difference at P < 0.05 by Tukey’s test.