Figure 4.

Effect of knockdown of HvNramp5 on plant growth at different Mn concentrations. A to C, Growth of wild-type barley (cv Golden Promise), the homozygous wild type (RNAi-wild type), and two homozygous transgenic RNAi lines (RNAi-1, RNAi-2) derived from two independent events. All lines were cultivated in a nutrient solution containing 0.05 (A), 0.5 (B), and 5 µm (C) Mn in the presence of 0.1 µm Cd for 14 d. D to F, Root length (D) and dry weight of roots (E) and shoots (F) in these lines were recorded at harvest. Data are means ± sd of three biological replicates and ** indicates significant difference at P < 0.01 by Tukey’s test.