Figure 8.

Comparison of HvNramp5 and OsNramp5 expression level in the root between barley and rice. The expression level of HvNramp5 and OsNramp5 in the roots was compared between barley (cv Golden Promise) and rice (cv Nipponbare) by absolute quantitative real-time PCR using common primers. Rice and barley were grown in nutrient solutions containing 0.05, 0.5, or 5 µm Mn for 7 d and then the roots were sampled for RNA extraction and gene expression analysis. Data are means ± sd of three biological replicates and ** indicates significant difference at P < 0.01 by Student’s t test.