Figure 3.

RIN binding to the regulatory regions of target genes revealed by EMSA. The promoter regions of the target genes are indicated. Red boxes indicate CArG-box elements in the promoter region, and numbers above represent the position of these motifs relative to the translational start site. The probe sequences used for EMSA for each target gene are shown, with red letters representing the CArG box. The protein-DNA complexes were separated on 6% native polyacrylamide gels. Triangles indicate the increasing amounts (10×, 100×, or 1000×) of unlabeled probes used for competition. The specific complexes formed are indicated by arrowheads. VI, Vacuolar invertase; VIF, vacuolar invertase inhibitor; LIN, cell wall invertase; CIF, cell wall invertase inhibitor; NI, cytoplasmic invertase.