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. 2016 Sep 30;172(3):1596–1611. doi: 10.1104/pp.16.01269

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Figure 9. — Quantitative proteomic analysis showing that ripening-related genes are affected by SlVIF expression. The changes in protein abundance in the SlVIF-silenced fruits (vif) at 38 DPA and 41 DPA are shown as the ratio between vif and the wild type (WT). The mRNA expression levels were measured by qRT-PCR. Total RNA was extracted from pericarp tissues of SlVIF silenced fruit (vif) and the wild type at 35 DPA, 38 DPA, 41 DPA, and 44 DPA. The gene transcript levels were normalized against the ACTIN gene, followed by normalization against the wild type at 35 DPA. The changes in transcript levels at 38 DPA and 41 DPA are shown. The results for both protein and mRNA expression are means ± sd from three independent experiments. PDS, Phytoene desaturase; CRTISO, carotenoid isomerase; ACO1, 1-aminocyclopropane-1-carboxylate oxidase; E4, peptide Met sulfoxide reductase; E8, 1-aminocyclopropane-1-carboxylate oxidase-like protein; SAM1, S-adenosyl-Met synthase 1; SAM3, S-adenosyl-Met synthase 3; LoxC, lipoxygenase; PAL, Phe ammonia-lyase; VI, vacuolar invertase; LePG, polygalacturonase A; MAN4, mannan endo-1,4-β-mannosidase; PME2.1, pectinesterase; EXPA4, expansin.