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. 2016 Sep 12;172(3):1451–1464. doi: 10.1104/pp.16.00585

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Figure 4. — Interaction of CpcG2 with NDH-1 and PSI complexes. A, Coimmunoprecipitation assay of the interaction of CpcG2 with NDH-1 and PSI complexes. Membrane proteins from the WT-CpcG2-YH and wild-type (WT) strains were incubated with protein A-Sepharose-coupled anti-GFP antiserum. The immunoprecipitates (IP) were probed with specific antibodies, as indicated on the left. B, Pull-down analysis of the interaction of CpcG2 with NDH-1 and PSI complexes. Membrane proteins isolated from the WT-CpcG2-YH and wild-type strains were purified using Ni²⁺ affinity chromatography. The purified samples were immunoblotted with antibodies against the subunits of the major photosynthetic membrane protein complexes. TM, Thylakoid membrane.