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. 2016 Sep 12;172(3):1451–1464. doi: 10.1104/pp.16.00585

Figure 5.

Figure 5.

The presence of CpcG2 is necessary for the stabilization of the NDH-1-PSI supercomplex. A and B, Coimmunoprecipitation assay of the interaction of NDH-1 with PSI. Membrane proteins from the wild-type (WT) and ΔcpcG2 strains were incubated with protein A-Sepharose-coupled antiserum of anti-NdhH (A) or anti-NdhK (B). The immunoprecipitates (IP) were probed with specific antibodies, as indicated on the left. TM, Thylakoid membrane. C and D, Freshly isolated thylakoid membranes from wild-type (C) and ΔcpcG2 (D) strains were solubilized in 1.5% DM at a Chl a concentration of 0.25 µg µL−1, and the protein sample was separated by 5% to 12.5% BN-PAGE. Furthermore, thylakoid membrane complexes separated by BN-PAGE were subjected to 12% SDS-PAGE, and the proteins were immunodetected with specific antibodies against NdhH and NdhK, respectively. Red arrows in the PSI (s) cluster represents band I. PSI (s), PSI supercomplex; PSI (t), PSI trimer; PSI (d), PSI dimer; PSI (m), PSI monomer.