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. 2016 Sep 27;172(3):1548–1562. doi: 10.1104/pp.16.00985

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Figure 1. — EIN3 stabilization during A/R. 35S::EIN3-GFP seedlings (7-d-old) in the ein3eil1 mutant background (35S::EIN3GFP-OE/dm) were used to follow EIN3 levels by immunoblot detection of EIN3-GFP under A/R treatment. Anoxia treatment was performed in the dark. Reoxygenation was carried out either in the dark (A) or light (B). Nor, Plants grown under aerated conditions (normoxia); Re0, after 4 h of anoxia (beginning of reoxygenation); Re1, Re3, and Re6, 1, 3, and 6 h of reoxygenation, respectively. Treatment with AgNO₃ (20 μm) or 1-methylcyclopropene (1-MCP) was used as a negative control to inhibit ethylene signal transduction. The quantified signal values were normalized to time point Nor and are shown below the gels.