Figure 7.

Changes of carbohydrates, α-tocopherol, and phytosterols in wild-type and gdh1gdh2 plants in response to A/R. GC-TOF-MS was applied to investigate carbohydrates, α-tocopherol, and phytosterols during A/R. Black lines with diamonds correspond to the wild type, and dotted lines with circles correspond to the gdh1gdh2 mutant. The x axis shows the treatment time course. The time point −4 corresponds to normoxia, and the zero point is set as the beginning of reoxygenation. The time points 1, 3, and 6 represent 1, 3, and 6 h of reoxygenation, respectively. The level of metabolites is expressed as relative fold change that is calculated by normalizing values of the wild type (normoxia). The results are mean values of at least four biological replicates with se. Asterisks indicate P < 0.05 by Student’s t test. F-6-P, Fru-6-P; G-6-P, Glc-6-P. Campesterol, stigmasterol, and sitosterol are common sterols in plants.