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. 2016 Oct 4;172(3):2033–2043. doi: 10.1104/pp.16.01254

Figure 3.

Figure 3.

Leaf phenotypes in THI2 knockdown plants and the LORE1 insertion mutants thiC and thi1. A and B, Expression of THI2 (A) and its paralog THI1 (B) in MG (control) and THI2KD plants 3 weeks after inoculation with rhizobia. THI2 expression in roots is shown in the inset. Expression levels were normalized to that of the ubiquitin gene. Error bars indicate sd (n = 3–5). Significant differences from MG plants are shown (Student’s t test: *, P < 0.05). C to L, LORE1 insertion thiC and thi1 mutants and THI2KD were either untreated (−T) or treated with thiamine at 50 mg L−1 (+T). Gifu, L. japonicus B129 Gifu (the wild type for LORE1 insertion mutants); MG, L. japonicus MG-20 Miyakojima (the wild type for THI2KD). Bars = 2 mm.