Figure 3.

Molecular and genetic identification of TWS1 as At5g01075. A, Schematics of the TWS1 locus. The TWS1 gene structure is represented by blue boxes for exons, white boxes for introns, and gray boxes for other sequences. The positions of the tws1-1 and tws1-2 insertion mutations are shown both in relation to the TWS1 start codon (in black), or to the chromosome 5 (Chr5) sequence (in gray). Sequenced T-DNA borders are indicated in red (RB, right border; LB, left border), ATG indicates the first codon. B, TWS1 protein sequence. The predicted signal peptide is underlined. C to H, tws1-1 complementation analysis. C, Wild-type mature dry seeds, (D) tws1-1 mature dry seeds, and (E) mature dry seeds produced by a tws1-1 plant transformed with a construct carrying the TWS1 coding sequence under the control of a double 35S promoter sequence. The picture shows the segregating progeny of a hemizygous plant. White stars, wild-type-looking seeds; black arrowheads, tws1-1-looking seeds. F to H, Hypocotyls SEM images of 7-d-old seedlings: F, wild type; G, tws1-1; and H, complemented tws1-1. Scale bars = 300 µm.