Seed content is perturbed in tws1 mutants. A, Total fatty acid quantification in wild-type, tws1-1, and tws1-2 seeds. B, Starch quantification in wild-type, tws1-1, and tws1-2 seeds. C, Suc quantification in wild-type, tws1-1, and tws1-2 seeds. A to C, *Values are significantly different from wild type (extractions performed on 30 dry seeds per genotype, on three biological replicates, Student’s t test, P < 0.05). D, SDS-PAGE gel run with crude seed protein extracts. The volumes corresponding to a quarter of a seed, half of a seed, and one seed have been loaded on the gel.