Figure 3.

Intracellular/extracellular H₂O₂ in guard cells of wild-type (WT), A2, and S2.2 plants post-NaCl treatment. A, Representative CLSM images of intracellular BES-H₂O₂-Ac fluorescence (green) and chlorophyll (Chl) autofluorescence (red) at 0, 1, and 6 h post-NaCl treatment. White boxes (black in merged images) denote nuclei. Images are representative of three independent experiments with six micrographs per genotype in each time point. Quantification of green signal is shown at right. Bars = 20 μm. B, CLSM images of intercellular BES-H₂O₂ fluorescence (green) and chlorophyll autofluorescence (red) at 0, 1, and 6 h post-NaCl treatment. White boxes (black in merged images) denote nuclei. Images are representative of three independent experiments with six micrographs per genotype in each time point. Quantification of green signal is shown at right. Bars = 20 μm. Data in charts at right are means ± se of three independent experiments with three technical replicates each. Different letters indicate significant differences of Duncan’s multiple comparisons (P < 0.05). RU, Relative units.