Figure 5.

mRNA levels and activity of NADPH oxidase in wild-type (WT), A2, and S2.2 leaves post-NaCl treatment. A, Abundance of mRNA levels of RbohD (left) and RbohF (right) in leaves post-NaCl treatment with 200 mm NaCl. B, Gel images showing the in gel activity assay of NADPH oxidase 1 h post-NaCl treatment with 200 mm NaCl. Images are representative of three independent experiments with one technical replicate in each (one gel). C, Quantification of anodal and cathodal isoenzymes of NADPH oxidase. A similar isoenzyme pattern has been reported previously in tobacco (Sagi and Fluhr, 2001). Data in A and C are means ± se of three independent experiments with three technical replicates. Different letters indicate significant differences of Duncan’s multiple comparisons relative to the wild type (P < 0.05). RU, Relative units.