Figure 6.

Intracellular/extracellular H₂O₂ in guard cells of wild-type (WT), AS-NtRbohD, and AS-NtRbohF plants post-NaCl treatment. A, CLSM images of intracellular BES-H₂O₂-Ac fluorescence (green) and chlorophyll (Chl) autofluorescence (red) at 0, 1, and 6 h post-NaCl treatment. White boxes denote nuclei. Images are representative of three independent experiments with six micrographs per genotype in each time point. Bars = 20 μm. B, CLSM images of intercellular AUR fluorescence (red) at 0, 1, and 6 h post-NaCl treatment. Images are representative of three independent experiments with six micrographs per genotype in each time point. Bars = 20 μm.