Table I. Properties of C. reinhardtii acclimated to different light and carbon supply regimes.
Changes in C. reinhardtii phenotype upon acclimation to different light and carbon supply regimes are shown. Data columns from left to right show cell number doubling time; Chl content per cell; Chl a/b ratio, calculated from the fitting of absorption spectra of 80% acetone-extracted pigments from cells; PSI/PSII ratio, measured based on the electrochromic shift (ECS) signal; PSI/PSII ratio, obtained by immunoblot (WB) quantification of PSAA and CP43; LHCII/PSII monomer calculations based on Chl a/b and PSI/PSII data, as described in “Materials and Methods”; and maximal quantum efficiency of PSII (Fv/Fm). The data are averages and se derived from a minimum of two biological replicates, each with three technical replicates.
| Condition | Doubling Time | Chl Content | Chl a/b | PSI/PSII ECS | PSI/PSII WB | LHCII/PSII | Fv/Fm |
|---|---|---|---|---|---|---|---|
| h | pg | ||||||
| Mnl | 9.0 ± 0.0 | 3.9 ± 0.21 | 2.57 ± 0.00 | 1.05 ± 0.34 | 1.00 ± 0.00 | 4.9 | 0.71 ± 0.01 |
| Mhl | 7.3 ± 0.4 | 2.0 ± 0.11 | 2.87 ± 0.03 | 1.13 ± 0.02 | 1.35 ± 0.30 | 4.3 | 0.70 ± 0.02 |
| Pnl | 25.0 ± 1.4 | 2.6 ± 0.02 | 2.55 ± 0.03 | 1.67 ± 0.11 | 1.95 ± 0.31 | 8 | 0.68 ± 0.01 |
| Phl | 22.5 ± 2.1 | 1.3 ± 0.02 | 2.57 ± 0.02 | 1.54 ± 0.58 | 1.65 ± 0.28 | 7.5 | 0.59 ± 0.00 |
| CO2nl | 8.8 ± 0.4 | 2.1 ± 0.12 | 2.56 ± 0.05 | 0.96 ± 0.04 | 1.04 ± 0.17 | 4.9 | 0.75 ± 0.01 |
| CO2hl | 4.6 ± 0.5 | 0.6 ± 0.02 | 3.00 ± 0.05 | 0.62 ± 0.04 | 0.66 ± 0.13 | 2.2 | 0.76 ± 0.01 |