Figure 5.
Tissue-specific expression of VuAAE3 in response to Al stress. A, Seedlings of rice bean (3 d old) were exposed to 0 or 25 µm Al for 8 h. Root tip (0–1 cm), basal root (1–2 cm), and leaves were sampled for RNA extraction. The expression was determined by real-time RT-PCR and 18S rRNA was used as an internal control. Values are expressed as means ± sd (n = 3). The asterisk indicates significant differences between treatments. B, Seedlings (7 d old) of transgenic Arabidopsis lines carrying PVuAAE3::GUS construct were subject to 1:30 strength Hoagland nutrient solution at pH 5.0 with or without 4 µm Al for 6 h. Bar = 100 µm.