Figure 3.
Activity of SlSFR2 in vitro. A, Immunoblot of yeast microsomes containing approximately equal amounts of recombinant His-tagged LacZ and SFR2 proteins. AtSFR2 and SlSFR2 microsomes with these comparable protein amounts were used for the assays. B, Activity comparison between AtSFR2 and SlSFR2 in a buffer with pH 7.5 and 8 mm MgCl2. Activity is shown as the molar ratio between the product DGDG and the substrate MGDG (n = 3, ±sd). C, Magnesium dependency of SFR2 activity. Assay buffers contained MgCl2 concentrations ranging from 0 to 12 mm (n = 3–4, ± sd). D, pH dependency of SlSFR2 activity. Assays buffers had a range of pHs from 6.72 to 8.26 (n = 3, ±sd).