Figure 4.

Generation the SlSFR2-RNAi lines. A, Simplified diagram of the SlSFR2-RNAi construct. RB, Right border; p35S, Cauliflower mosaic virus (CaMV) 35S promoter; Intron, pyruvate dehydrogenase kinase (PDK) intron; Tnos, NOS-terminator; LB, left border. B, Quantitative RT-PCR probing SlSFR2 mRNA abundance in wild-type (SlWT) and SlSFR2-RNAi lines. C, TLC of the lipids from Arabidopsis wild-type plants (AtWT), tomato wild-type plants (SlWT), or select transgenic lines (SlL2 and SlL29). Lipids were stained for sugar head groups. The leaves were incubated in either water or 0.5 m MgCl₂ aqueous solution for 6 h before lipid extraction.