Skip to main content
. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Cancer Res. 2016 Aug 16;76(19):5822–5831. doi: 10.1158/0008-5472.CAN-16-1019

Figure 4. Effect of RAPTOR and p21 shRNA.

Figure 4

A) 8226 cells expressing shRNA targeting a scrambled sequence (control) or two separate sequences of RAPTOR, followed by immunoblot assay; B) MTT assay of control (SCR-control) or RAPTOR-silenced 8226 MM cells (shRaptor1 and 2) challenged with NSC 126405 for 48 or 72 hrs. Data are mean+/−SD, n=3. * denotes significant difference (p<0.05) from SCR control cells. Apoptosis (mean+/−SD) is shown above 2 & 4 uM bars in the 72 hrs assay; C) 8226 cells expressing shRNA targeting scrambled sequence or two separate sequences of p21, followed by immunoblot assay; D) Cell cycle distribution of control (SCR) or p21-silenced MM cells (Shp21-1 & Shp21-2) exposed to 0, 1 or 2 uM NSC 126405 for 48 hrs. * denotes significant difference (p<0.05) from control (0 uM).