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. Author manuscript; available in PMC: 2016 Nov 8.
Published in final edited form as: Arch Neurol. 2012 Sep;69(9):1125–1131. doi: 10.1001/archneurol.2012.1300

Figure 2.

Figure 2

Aquaporin 4281–300 is the immunodominant linear determinant of human aquaporin 4 (hAQP4). HLA-DRB1*03:01 transgenic mice were immunized with a full-length hAQP4 complete Freund adjuvant emulsion, resulting in each mouse receiving 100 µg of antigen. On day 10, cells taken from the lymph nodes (A) and spleens (B) were collected from mice to generate single-cell suspensions. Thereafter, cells (0.5 × 106 cells) were incubated for 48 hours in 96-well enzyme-linked immunosorbent spot assay plates coated with anti–interferon-γ (IFN-γ) or anti–interleukin-17 (IL-17) with single overlapping hAQP4 peptides (50 µg/mL) (Table 1), media only, full-length hAQP4 (50 µg/mL), or concanavalin A (ConA) (1 µg/mL). Spot-forming units represent the absolute number of cells that are secreting a specific cytokine in response to antigen in the well (*P < .001). Error bars indicate SE.