Skip to main content
. Author manuscript; available in PMC: 2016 Nov 8.
Published in final edited form as: Arch Neurol. 2012 Sep;69(9):1125–1131. doi: 10.1001/archneurol.2012.1300

Figure 4.

Figure 4

Aquaporin 4281–300 (AQP281–300) does not inhibit AQP4 antibody binding. AQP4-specific and isotype control recombinant antibodies (rAbs) were incubated with a U87MG glial cell line stably transfected with the M23 isoform of human AQP4 in the presence or absence of AQP4 peptide. A, Fluorescence micrographs demonstrate robust binding of AQP4-specific rAbs 53 and 58 (green) to the transfected cell line. No staining was observed in the absence of rAb (secondary control) or when using an isotype control rAb (ON7-5 No. 51). A rabbit polyclonal anti-AQP4 antibody against the intracellular C-terminal portion of AQP4 was used as an internal control for AQP4 expression. B, Percentage binding of AQP4-specific rAbs 53 and 58 in the presence and absence of AQP4 peptides. NMO indicates neuromyelitis optica. Error bars indicate SE.