Figure 3. Nr5a2 is not required for the formation of pdx1 and ptf1a pancreas progenitors.

A) The area of pdx1 pancreas progenitors is similar across nr5a2^+/+, nr5a2^+/oz3, and nr5a2^oz3/oz3 embryos at 26 hpf.

B) No significant differences in pdx1 progenitor population size identified by a quantification of pdx1 expression area by genotype (p > 3 per group; for nr5a2^+/+ vs. nr5a2^+/oz3, p = 0.6746; for nr5a2^+/+ vs. nr5a2^oz3/oz3, p = 0.6606; unpaired t-test).

C) pdx1 pancreas progenitors are formed at 48 hpf in nr5a2^+/oz3 and nr5a2^oz3/oz3 embryos; however, subtle difference in pdx1 progenitor budding is noted with variable penetrance across the nr5a2^oz3/oz3 population.

D) No significant differences in the size of the pdx1 pancreas progenitor pool were observed by quantification of expression area at 48 hpf (n > 3 per group; for nr5a2^+/+ vs. nr5a2^+/oz3, p = 0.6024; for nr5a2^+/+ vs. nr5a2^oz3/oz3, p = 0.4178; unpaired t-test).

E) ptf1a progenitors are formed in nr5a2^+/oz3 and nr5a2^oz3/oz3 embryos by 48 hpf.