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. Author manuscript; available in PMC: 2016 Nov 8.
Published in final edited form as: Methods Mol Biol. 2009;543:563–576. doi: 10.1007/978-1-60327-015-1_33

Figure 2.

Figure 2

Analysis of chromatin templates by native PAGE. Chromatin was assembled on the radioactively labeled 2.5 kb ApoI/EcoRI pYP05 fragment at the indicated mass ratios of donor chromatin to template DNA. Chromatin assembly results in a progressive decrease of the mobility of the complexes in the gel. Chromatin reconstituted at 1:2 ratio of the donor-chromatin to DNA is saturated (S), since further increase in the ratio up to 1:1 does not result in further shift of the corresponding band. Saturated chromatin assembled at 1:2 ratio was used in the transcription experiment (Fig. 5).