Figure 3.

The effect of ribosome density on GAP1 mRNA degradation rate. (A) Mutation of the wild-type start codon in GAP1 from ATG to GTG results in (A) a reduced steady state transcript level, and (B) an increased mRNA degradation rate. In (B) we show the average value for each time point ± SEM. In (C) we show bootstrapped CI for the regression of all data points. Solid lines indicate the line of best fit, and dotted lines indicate CI. In blue is the transcript with a wildtype start codon. In red is the transcript with a mutated start codon.