Table 3. Reduction of phosphocholine synthesis via pmt RNAi does not reduce LET-23 activity in vulval development or uv1 cell specification.
| No. of Vulval Invaginations L4 Stage | No. of GFP-Positive uv1 Cells | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | n | Average | 4 | 5 | 6 | n | Average | |
| Wild typea | 50 | 0 | 0 | 0 | 50 | 1.00 | 30 | 0 | 0 | 30 | 4.00 |
| let-23(sa62gf)b | 2 | 21 | 10 | 1 | 34 | 2.29 | 49 | 8 | 1 | 58 | 4.17 |
| let-23(sa62gf); pmt-1 RNAi | 0 | 13 | 37 | 8 | 58 | 2.91* | 32 | 8 | 0 | 40 | 4.20 |
| let-23(sa62gf); pmt-2 RNAi | 0 | 8 | 10 | 0 | 18 | 2.56 | 36 | 11 | 2 | 49 | 4.31 |
P < 0.05, calculated using Student’s t-test.
Strain BL5715.
Strain HV760.