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. 2016 Sep 8;6(11):3647–3653. doi: 10.1534/g3.116.033266

Figure 1.

Figure 1

Multiplex gene targeting in P. patens PpKAI2L clade i gene family using a CRISPR-Cas9 system without selection pressure. (A) Percentage of single and multiple mutants obtained in experiments I (n = 63) and II (n = 26), targeting the PpKAI2L genes of clade i. Experiment I uses a mix of four sgRNA plasmids targeting PpKAI2L-A to –D. Experiment II uses a mix of five sgRNA plasmids targeting PpKAI2L-A to -E. (B) Distribution of deletion, insertion, and insertion-deletion (In & Del) ontarget events found for each sgRNA in experiment I. (C) Distribution of deletion, insertion, and insertion-deletion (In & Del) on-target events found for each sgRNA in experiment II.