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. 2016 Sep 16;6(11):3663–3670. doi: 10.1534/g3.116.034363

Figure 6.

Figure 6

Downregulation of the LIN-12 intracellular domain requires assembly of the nuclear complex. (A) Analysis of mutations affecting the RAM domain. The RAM domain contains a tetrapeptide site required for LAG-1/CSL to bind to LIN-12(intra) and subsequently form a nuclear complex (Wilson and Kovall 2006). Deletion of the RAM+ANK or mutation of the LAG-1/CSL binding site prevent LIN-12(intra) from associating with the nuclear complex, which abrogates LIN-12(intra) activity and stabilizes LIN-12(intra) protein in the VPC and VPC descendants [Pn.p (VPCs), Pn.px (their daughters), and Pn.pxx (their granddaughters)]. (B) Photomicrographs showing stabilization resulting from mutation of the RAM domain. CPD, Cdc4 phosphodegron; GFP, green fluorescent protein.