Figure 3.

Maternal contributions of Cas9 and GAL4-targeting gRNAs lead to inherited mutations in GAL4. Male progeny of the four genotypes shown in Figure 2B were crossed to UAS-mtdT3xHA + QUAS-6xGFP reporters to determine the extent of germline GAL4 alterations. The percentage of progeny from this cross that contained functional GAL4 (GAL4+), disrupted GAL4 (dGAL4), or HDR-mediated functional QF2 (QF2+) were calculated. Maternal contribution of Cas9 components in the developing paternal germline cells (far right column) led to 87% of progeny being mutant for GAL4. GAL4 was almost completely disrupted when male genotypes contained Cas9 components (far left column). Genomic expression of gRNAs vs. Cas9 was more potent at increasing the effects of maternal contributions.