Fig 2.

Effect of SIRT6 depletion on tumor growth in a xenograft mouse model (A) Hep3B cells were transduced with a lentiviral vector containing NC- or SIRT6-shRNA sequences. Hep3B cells were harvested 4 days after viral transduction, and 1 × 10⁶ cells were subcutaneously injected into mice. Tumor sizes in 21 mice were measured every 4 days after the injection and are shown as means ± SEM. (B) Photographs of mice injected with Hep3B cells were taken after sacrifice. (C) Tumors were obtained from the sacrificed mice, and their sizes were compared. (D) Tumor weights from sacrificed mice are shown as means ± SEM. (E) The expression of Ki-67 was immunohistochemically examined by confocal microscopy, and Ki-67-positive cells were counted in randomly obtained images. The scale bar indicates 50 μM. (*P<0.01, **P<0.05).