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. 2016 Nov 8;11(11):e0165835. doi: 10.1371/journal.pone.0165835

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© 2016 Lee et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — Induction of cellular senescence by SIRT6 depletion in the p16/Rb and p53/p21- independent manners (A) Representative micrographs of Hep3B cells 6 days after infection with lentiviruses containing NC or shSIRT6. The scale bar indicates 20 μM. (B) Representative micrographs of SA-ß-gal staining in NC and shSIRT6-depleted Hep3B and Huh-7 cells. The percentage of SA-ß-gal-positive cells was quantified from 6 different areas in the plate. (C) mRNA levels of the selected cytokine genes in control and SIRT6-silenced Hep3B cells are indicated in the histogram. (D) The expression of p16, Rb, p53, and p21 was analyzed by western blotting. Three independent experiments were performed. (**P<0.01).