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. 2016 Nov 8;11(11):e0165506. doi: 10.1371/journal.pone.0165506

Fig 5. Effect of different blood dilutions on the RT-LAMP signals.

Fig 5

One hundred nanograms of total RNA or 2.5 μL of lysed samples from differently diluted (1/2 down to 1/50 in lysis buffer) P. falciparum-parasitized blood samples were tested by RT-LAMP. A) RT-LAMP amplification profiles showing drastic drops in maximum fluorescence intensities with increasing concentration of lysed blood components. B) Visual appreciation of GelGreen fluorescence showing masking of fluorescence intensity with increasing concentration of lysed blood components. Whole blood dilutions were as follows; II: 1/50, III: 1/20, IV: 1/10 in 1x lysis buffer, and V: 1/10, VI: 1/5, VII: 2/5, VIII: 1/2 in 2x lysis buffer.