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. 2016 Nov 8;11(11):e0165964. doi: 10.1371/journal.pone.0165964

Fig 3. Overexpression of Syn87PEST and VH14PEST reduced total cell death to control levels in ST14A cells.

Fig 3

(A) VH14PEST reduced the percentage of total cell death to a greater extent than Syn87PEST. [Mean ± SEM. * p<0.05 compared to Syn-GFP; † p<0.05 compared to Syn87PEST; n = 3]. (B & C) Representative scatter plots showing the percentage of cells in each quadrant. Viable cells are annexin V (−) and 7-AAD (−); annexin V (+) and 7-AAD (−) cells are in early apoptosis; annexin V (+) and 7-AAD (+) cells are in late apoptosis; necrotic cells are annexin V (−) and 7-AAD (+). (B) The percent total of cells undergoing late apoptosis was significantly reduced by VH14PEST compared to empty vector control co-transfected GFP levels. (Mean ± SEM. *p<0.05 comparing control GFP, to other groups; ᵻ p<0.05 compared VH14PEST/SYNGFP to other groups; n = 3). (C) The percent Annexin V positive cells undergoing early apoptosis was significantly reduced to empty vector control co-transfected GFP levels. (Mean ± SEM. *p<0.05 comparing control GFP, VH14PEST Syn-GFP to other groups; n = 3).