Fig 5. DBT induces the expressions of anti-oxidative enzymes.

Cultured H9C2 cells were treated with a series of chemically modified DBT decocton (0.5–2.0 mg/mL) or ferulic acid (809–5000 ng/mL) for 24 hours (A-E). The cell lysates were collected to determine the protein expressions of NQO1 and GCLM using specific antibody. Five μM of tBHQ served as positive control. GAPDH served as loading control. Quantification of protein amount from the blot was calculated by a densitometer (F). Values were expressed as the fold of increase to basal reading (untreated culture). Data were expressed as mean ± SEM, where n = 3. *** p < 0.001, ** p < 0.01, * p < 0.05 as compared to the control.