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Phosphorylation of myelin basic protein (P-MBP) on PKA substrate motifs is increased with HIV infection of the brain. Myelin basic protein was immunoprecipitated and analyzed using the anti-phospho serine/threonine PKA substrate primary antibody. Lanes 1–4, brain lysates from healthy individuals; lanes 5–6, brain lysates from individuals with HIV infection without encephalitis; lanes 7–8, brain lysates from individuals with HIV infection and encephalitis.
