Table 1.
Novel phosphorylation sites discovered in the human brain.a
| Sequence | Protein | UniProt Accession | Phospho site | Corresponding motif | Kinase Substrate motif | Binding motif | Binding domain |
|---|---|---|---|---|---|---|---|
| 1473KTEVQAHSPSR1483 | Microtubule-associated protein 2 | P11137 | S1480 | XXpSP, X[pS/pT]P, pSX[E/pS*/pT*] [pS/pT]PX[R/K], pSP, pSPX[R/K]X |
GSK3, ERK1, ERK2, CK II CDK1, 2, 4, 5, 6, Cdc2 Growth associated histone HI kinase |
[pS/pT]P | WW |
| 384GKVPVTYLELLS395 | SH3-domain GRB2-like endophilin B2 | Q9NR46 | S395 | [E/D]XX[pS/pT] | CK I | na | na |
| 255NVPATPPR262 | SH3-domain GRB2-like (endophilin) interacting protein 1 |
Q9BQI5 | T259 | PX[pS/pT]P, PX[pS/pT]PP X[pS/pT]P, X[pS/pT]XXX[A/P/S/T] [pS/pT]PX[R/K], PXpTP |
GSK3, ERK1, ERK2, CDK5 G protein-coupled receptor kinase 1 CDK1, 2, 4, 6, Cdc2 kinase, Growth associated histone HI kinase |
[pS/pT]P | WW |
| 210EPSLHEIGEK219 | fibroblast growth factor 12 | P61328 | S212 | [M/I/L/V/F/Y]XRXX[pS/pT] [M/I/L/V/F/Y]X[R/K]XX[pS/pT] [M/I/L/V/F/Y]XRXX[pS/pT][M/I/L/V/F/Y] [M/V/L/I/F]X[R/K]XX[pS/pT]XX [M/V/L/I/F]XRXX[pS/pT]XXX[M/V/L/I/F] RXXpS, RXX[pS/pT] [R/K]XX[pS/pT], R[K/E/R]XpS [E/D]X[pS/pT][I/L/V/M]X[E/D] P[pS/pT]X, pSXX[E/D] pSXX[E/pS*/pT*], [pS/pT]XX[E/D] [pS/pT]XX[E/D/pS*/pT*] |
Chk1 kinase Calmodulin-dependent protein kinase I, II, IV PKA, PKC, Plk1 kinase, CK II DNA dependent Protein kinase |
RXXpS | 14-3-3 |
| 921RGSPEEAAEREPR933 | calcium channel, voltage-dependent, N type, alpha 1B subunit | Q00975 | S923 | RXXpS, RXX[pS/pT], [R/K]X[pS/pT], RXpS, XXpSP, X[pS/pT]P, X[pS/pT]XXX[A/P/S/T], pSP, pSX[E/pS*/pT*], pSXX[E/D], pSXX[E/pS*/pT*], [pS/pT]XX[E/D], [pS/pT]XX[E/D/pS*/pY*] | PKC, PKA, GSK-3, ERK1, ERK2, CDK5, G-protein coupled receptor kinase 1, CK II, Calmodulin dependent protein kinase | RXXpS, [pS/pT]P | 14-3-3, WW |
| 214(S)(S)PAFGDRR222 | aminolevulinate dehydratase | P13716 | S214 or S215 | [R/K]X[pS/pT], X[pS/pT]P, XXpSP, pSP | PKA, PKC, GSK-3, ERK1/2, CDK5 | S[pS/pT]X, [pS/pT]P | MDC1 BRCT, Plk1 PBD, WW |
| 97ANCSPEDPCQETVSKPEVSK116 | proline rich transmembrane protein | Q7Z6L0 | S100 | KXXX[pS/pT], XXpSP, X[pS/pT]P X[pS/pT]XXX[A/P/S/T] XpSXXDXX, pSP pSX[E/pS*/pT*], pSXX[E/D] [pS/pT]XX[E/D] [pS/pT]XX[E/D/pS*/pT*] |
PKA, GSK-3, ERK1, ERK2, CDK5, G protein-coupled receptor kinase1, Pyruvate dehydrogenase kinase, ERK1/2, CKII | [pS/pT]P | WW |
| 793TEEERTPNHDGGK805 | neuronal cell adhesion molecule | P13591 | T798 | [E/D]XX[pS/pT], X[pS/pT]P, [R/K][pS/pT]P | Casein kinase I, GSK-3, ERK1, ERK2, CDK5, Growth associated histone HI kinase | [pS/pT]P | WW |
| 361NSAAATSPK369 | Ca2+/calmodulin-dependent protein kinase | Q13554 | S367 | XXpSP, X[pS/pT]P pSP, [pS/pT]P[R/K], [pS/pT]X[R/K], pSPXX[pS*/pT*] [pS/pT]XXX[S/T][M/L/V/I/F] |
GSK-3, ERK1/2, CDK5, PKA, PKC, CK I, Growth associated histone HI kinase | [pS/pT]P | WW |
| 185QTGNTCK191 | synaptophysin | P08247 | T186 | [R/K]X[pS/pT], [pS/pT]XX[S/T] | PKA, PKC, CK I | na | na |
| 297SGSPMAR303 | myelin basic protein | P02686 | S299 | pSXXX[pS/pT], pSXXXpS* RXXpS, RXX[pS/pT], [R/K]XX[pS/pT], XXpSP, X[pS/pT]P, pSP |
MAPKAPK2, GSK3, ERK1, ERK2, CDK5, Calmodulin-dependent protein kinase II, PKA, PKC | RXXpS, [pS/pT]P | 14-3-3, WW |
| 372G(S)(S)GVGL(T)AAVTTDQETGERR392 | DNA replication licensing factor MCM3 | P25205 | S373, S374 or T379 | X[pS/pT]XXX[A/P/S/T] | G-protein coupled receptor kinase1 | na | na |
| 137SRTAAYNTLK146 | V-type proton ATPase subunit C 2 | Q8NEY4 | Y142, T144 | [pS/pT]X[R/K], pYXX[L/I/V] | PKA, PKC, JAK2 | na | na |
| 16GWSTDEANTYFK27 | intelectin 1 | Q8WWA0 | Y25 | Na | na | na | na |
| 304(T)I(T)QKVLQK312 | olfactory receptor 10A7 | Q8NGE5 | T304 or T306 | [M/I/L/V]X[R/K]XX[pS/pT], [M/I/L/V/F/Y]XRXX[pS/pT], [M/V/L/I/F]X[R/K]XX[pS/pT]XX, [M/V/L/I/F][R/K/H]XXX[pS/pT]XXX[M/V/L/I/F], [pS/pT]X[R/K], [M/V/L/I/F]XRXX[pS/pT]XXX[M/V/L/I/F], [R/K]X[pS/pT], [R/K]XX[pS/pT] KXXX[pS/pT], RXX[pS/pT] [R/K]XX[pS/pT]X[R/K] |
Chk1 kinase, PKA, PKC, AMP-activated protein kinase Calmodulin-dependent protein kinase I, II, IV |
na | na |
| 1778EQASSLSDDRK1784 | Dynein heavy chain 3, axonemal | Q8TD57 | S1784 | pSXX[E/pS*/pT*] pSX[E/pS*/pT*] |
CK II | na | na |
| 628MKEPDGKLSPPK639 | teashirt zinc finger homeobox 3 | Q63HK5 | S636 | [R/K]X[pS/pT], XXpSP X[pS/pT]P, pSP [pS/pT]PX[R/K], pSPX[R/K]X |
PKA, PKC, GSK-3, ERK1/2, CDK1, 2, 4, 5, 6, Growth associated histone HI kinase, Cdc2 kinase | [pS/pT]P | WW |
a
Phosphorylated residues are underlined. Phosphosites with ambiguous localization appear in parenthesis. The phosphosites observed on these peptides are represented in bold on the motif, while the sites that are required to be phosphorylated first for the recognition of the motif are indicated with an asterisk. na: information not available.