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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: J Immunol. 2016 Sep 26;197(9):3639–3649. doi: 10.4049/jimmunol.1600402

Figure 3. Hif-2α deficiency enhances MARCO expression in primary mouse Mϕs, which is required for enhanced efferocytosis.

Figure 3

(A) qPCR data of indicated receptors from Hif-2αfl/fl LysMcre (TOP) vs after Phd3 siRNA from Mϕs under normoxic conditions. (B) Immunoblot of MARCO in Hif-2αfl/fl vs. Hif-2αfl/fl LysMcre Mϕs under normoxia. (C) Immunoblot of MARCO after PhD3 siRNA in Mϕs under normoxia. (D) Densitometry of MARCO protein after adenoviral Hif-2α expression under normoxia. (E) Cell surface flow cytometry of MARCO in Hif-2αfl/fl vs. Hif-2αfl/fl LysMcre Mϕs. (F) Hif-2αfl/fl versus Hif2αfl/fl LysMcre (CRE) primary Mϕs were treated with 10μg/mL MARCO blocking antibody (clone ED31, azide-free) or IgG1 isotype (ISO) control prior to co-culture with fluorescently-labelled apoptotic cells and efferocytosis enumerated. * indicates p < 0.05 vs. ISO control and # indicates p < 0.04 vs. CRE/ISO control. (G) Marco−/− Mϕs were treated with Hif-2α siRNA and challenged with apoptotic cells (Bar graph axis same as in (F).